3/15/2024 0 Comments Fiji imagej convert jpg to pngSo if you want to get into scripting - here is a helpful Scripting workshop and corresponding slides to get you started. When the Save as dialog is opened, Fiji will enter the image window’s name, plus the appropriate file suffix, as the File Name'. Other formats are available (see menu image on the right) and can be accessed by File/Save As. See if that does it… it can be adapted as you need. 4 Bio-Formats Image formats The File/Save (hotkey: S) menu command will save the image as a TIF file. SaveAs("Tiff", output+ "/" + nameOnly + "(RGB).tif") NameOnly = substring(imageName, 0, length-4) // this just cuts off the. You’ll need the image and path for where to save the file as well - so you can ask for both using a Script Parameters… the script would then look something like: File(label="Select an image file") = "Output directory", style = "directory") output If you use the Macro Recorder as a test - you can then see the code call you need. Use saveAs(format) to have a “Save As” dialog displayed. The format argument must be “tiff”, “jpeg”, “gif”, “zip”, “raw”, “avi”, “bmp”, “fits”, “png”, “pgm”, “text image”, “lut”, “selection”, “results”, “xy Coordinates” or “text”. Saves the active image, lookup table, selection, measurement results, selection XY coordinates or text window to the specified file path. The definition for that function is as follows: Image analysis is interdisciplinary, so clearly explain field-specific terms or jargon.Built In Macros Functions list is your friend. Clearly explain what you are trying to learn, not just the method used, to avoid the XY problem. Provide details: Be thorough in outlining the question(s) that you are trying to answer.People from the future may be stuck trying to answer the same question. Report spam or content that is hateful or off-topic.Upvote those who contribute to the discussion and provide freely of their time to assist you.Projects: Share a Link to your pet image analysis project.Research: Links to published (articles in scientific journals or in established repositories) that utilize ImageJ/FIJI for image analysis or are about image analysis.Discussions: Text posts, meant to ask about general issues relating to image analysis.Image analyst job posts are also welcome. Tips: Text or Link posts to share useful how-to tricks and discoveries on using ImageJ/FIJI.Questions which have been Solved will be marked as such. This could include algorithms, microscopy and scientific imaging, plug-ins, methods, and specific features of the software. Questions: Text posts asking about image analysis and ImageJ/FIJI.How important it is to remove background and all the image correction is really necessary? Thanks! Is it okay if I choose different parameters on different stain? 4. Should I normalize measurements like on dapi? But I feel like it's intensity is different in each picture. Should I select every time the same- same parameters for each picture? 2. Please tell me if I did anything wrong? And the questions are: 1. I don't have seperate cells its more like a monolayer and staining is poor so I didn't wanted to select seperate cells. I dublicated the pic, selected threshold and combined with the one I made dublucate from, measured the whole pic. Fiji bundles together many popular and useful ImageJ plugins for image analysis into one installation, and automatically manages their dependencies and updating. Then I imported lsm, enhanced contrast and measured on first given channel colormarked supposedly my target protein (the second one was definitely dapi stain). I've made measurements in tif at first, after splitting channels, but on next picture realized that I don't know how to deal with yellow color. So, I have pics from microscope in lsm and tif formats both. I can't figure out how to conceptually make measurements of fluoresence in my cell samples. Sorry for stupid questions I've been trying this program for a few days.
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